When you move the slide away from you on the stage, in what direction does the image appear to move?

Slide Motion
Describe the movement of the slide as you move the slide toward/ away from you. Describe the movement of the slide as you move the slide from side to side.

Looking at the slide, when the slide moves toward me, the image would seem to move away. Vice versa when the slide moves away, it would come towards me. Similarly, when the slide was moved from right to left, the image would go to the opposite side. So going right the image would seem to go left, and when the slide was moved to the left the image would go to the right.

Relationship
Describe the relationship between the direction the stage/slide is moved and the direction of movement in the field of view?

There is an inverse relationship between the directions slide is moved and direction of movement in the field of view. As explained in the previous question, when the slide was moved either towards or away, and side-to-side it would appear to go to the opposite side. Therefore, the relationship is formed between both direction of slide and direction of movement in fov.

Focus
Why do some parts of the specimen appear to be in focus when other parts are not?

Some parts of the specimen do not appear in focus because the specimen is not equally proportioned, some parts might be smaller and/ or not as equally thick. In

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Once finished turn the nose piece to the lowest magnification (lowest objective lens). Use coarse adjustment knob to lower stage as low as possible. Remove slide, clean lens if they became dirty and clean any debris off from stage. Make sure the stage clips are moved all the way to the right and the stage is moved all the way back or (flushed) to the front. Turn the light to the lowest light intensity and turn off. Unplug and wrap the cord on the back. If cover is available place over the microscope, now it is properly set back to

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  • Learning Objectives

    • Compare and contrast light and electron microscopy.

    Cells vary in size. With few exceptions, individual cells cannot be seen with the naked eye, so scientists use microscopes (micro- = “small”; -scope = “to look at”) to study them. A microscope is an instrument that magnifies an object. Most photographs of cells are taken with a microscope; these images can also be called micrographs.

    The optics of a microscope’s lenses change the orientation of the image that the user sees. A specimen that is right-side up and facing right on the microscope slide will appear upside-down and facing left when viewed through a microscope, and vice versa. Similarly, if the slide is moved left while looking through the microscope, it will appear to move right, and if moved down, it will seem to move up. This occurs because microscopes use two sets of lenses to magnify the image. Because of the manner by which light travels through the lenses, this system of two lenses produces an inverted image (binocular, or dissecting microscopes, work in a similar manner, but they include an additional magnification system that makes the final image appear to be upright).

    Light Microscopes

    To give you a sense of cell size, a typical human red blood cell is about eight millionths of a meter or eight micrometers (abbreviated as eight μm) in diameter; the head of a pin of is about two thousandths of a meter (two mm) in diameter. That means about 250 red blood cells could fit on the head of a pin.

    Most student microscopes are classified as light microscopes. Visible light passes and is bent through the lens system to enable the user to see the specimen. Light microscopes are advantageous for viewing living organisms, but since individual cells are generally transparent, their components are not distinguishable unless they are colored with special stains. Staining, however, usually kills the cells.

    When you move the slide away from you on the stage, in what direction does the image appear to move?
    Figure: Light and Electron Microscopes: (a) Most light microscopes used in a college biology lab can magnify cells up to approximately 400 times and have a resolution of about 200 nanometers. (b) Electron microscopes provide a much higher magnification, 100,000x, and a have a resolution of 50 picometers.

    Light microscopes, commonly used in undergraduate college laboratories, magnify up to approximately 400 times. Two parameters that are important in microscopy are magnification and resolving power. Magnification is the process of enlarging an object in appearance. Resolving power is the ability of a microscope to distinguish two adjacent structures as separate: the higher the resolution, the better the clarity and detail of the image. When oil immersion lenses are used for the study of small objects, magnification is usually increased to 1,000 times. In order to gain a better understanding of cellular structure and function, scientists typically use electron microscopes.

    Electron Microscopes

    In contrast to light microscopes, electron microscopes use a beam of electrons instead of a beam of light. Not only does this allow for higher magnification and, thus, more detail, it also provides higher resolving power. The method used to prepare the specimen for viewing with an electron microscope kills the specimen. Electrons have short wavelengths (shorter than photons) that move best in a vacuum, so living cells cannot be viewed with an electron microscope.

    In a scanning electron microscope, a beam of electrons moves back and forth across a cell’s surface, creating details of cell surface characteristics. In a transmission electron microscope, the electron beam penetrates the cell and provides details of a cell’s internal structures. As you might imagine, electron microscopes are significantly more bulky and expensive than light microscopes.

    Key Points

    • Light microscopes allow for magnification of an object approximately up to 400-1000 times depending on whether the high power or oil immersion objective is used.
    • Light microscopes use visible light which passes and bends through the lens system.
    • Electron microscopes use a beam of electrons, opposed to visible light, for magnification.
    • Electron microscopes allow for higher magnification in comparison to a light microscope thus, allowing for visualization of cell internal structures.

    Key Terms

    • resolution: The degree of fineness with which an image can be recorded or produced, often expressed as the number of pixels per unit of length (typically an inch).
    • electron: The subatomic particle having a negative charge and orbiting the nucleus; the flow of electrons in a conductor constitutes electricity.

    When you move the slide to the right in what direction does the image move?

    9. The proper way to use a microscope is to look through the eyepiece with both eyes open to help avoid eye strain. Remember, everything is upside down and backwards. When you move the slide to the right, the image goes to the left!

    When you move the slide forward on the stage in what direction does the E appear to move when viewed through the microscope?

    - The letter “e” - The viewing of this familiar letter will provide practice in orienting the slide and using the objective lenses. The letter appears upside down and backwards because of two sets of mirrors in the microscope.

    When the stage is moved away from you which direction does the E on the slide move as you watch it through the ocular lens?

    Describe the orientation of the letter "e" as it appears through the ocular lenses. The image of the "e" appears backward and upside down. As you move the slide toward the right of the stage, to which direction does the image of the "e" move when viewed through the microscope? To the left.

    When you move the slide from left to right at which direction does the image in the microscope move to?

    The image moves in the opposite direction. If the slide moves to the left, the image is moved to the right. If the slide is moved to the right, the image is moved to the left.