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What is the problem in determining rate at low substrate concentration?For low substrate concentrations (relative to the Km), depletion of the substrate causes the reaction to slow down more than at higher substrate concentration, so a low enzyme concentration is needed to maintain the initial rate long enough for the initial rate measurement to be made.
How does initial concentration of substrate affect rate of enzyme action?Initially, an increase in substrate concentration leads to an increase in the rate of an enzyme-catalyzed reaction. As the enzyme molecules become saturated with substrate, this increase in reaction rate levels off. The rate of an enzyme-catalyzed reaction increases with an increase in the concentration of an enzyme.
Why does substrate concentration reach a maximum reaction rate?At low substrate concentration the reaction rate increases sharply with increasing substrate concentration because there abundant free enzyme available (E) to bind added substrate.
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4. explain why the maximum initial reaction rate cannot be reached at low substrate concentrations.
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